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. 2023 Dec 29;11(2):uhad295. doi: 10.1093/hr/uhad295

Figure 3.

Figure 3

PM resistance in CRISPR/Cas9 mutants of CsMLO8/11. a, b Schematic illustrating the gene structure of (a) CsMLO8 and (b) CsMLO11; filled blue, yellow boxes, and black lines represent the UTR, exons, and introns respectively. mlo8CR1 and mlo8CR2 are homozygous mutants for CsMLO8. mlo11CR1 and mlo11CR2 are homozygous mutants for CsMLO11. c–f PM infection phenotype of mutants (mlo8CR1, mlo8CR2, mlo11CR1, mlo11CR2, mlo8CR1mlo11CR1) and WT plants in (c) stem, (d) hypocotyl, (e) adult leaf, and (f) young leaf. Photographs were taken at 62 dps for the stem, 8 dpi for the hypocotyl, 55 dps for the adult leaves, and 6 dpi for the young leaves. g–j Statistical analyses of DI for (g) stem, (h) hypocotyl, (i) adult leaf, and (j) young leaf. ***P < 0.001; **P < 0.01; *P < 0.05, Student’s t-test, n = 3. k–n Number of spores in (k) stem, (l) hypocotyl, (m) adult leaf, and (n) young leaf. ***P < 0.001, Student’s t-test, n = 6. o Trypan blue staining of young leaves of the WT and mlo mutants at 6 dpi. Red arrows indicate spore hyphae. Scale bars, 50 μm.