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GPNMB ameliorates spinal cord tissue damage in rats after SCI. H&E (A) and Nissl (B) staining were used to evaluate the extent of spinal cord tissue damage in rats. TUNEL staining (C) was performed to assess neuronal apoptosis. Immunofluorescence staining of NeuN (D) was conducted to evaluate neuronal survival. DAPI: 4′,6-diamidino-2-phenylindole; H&E: hematoxylin and eosin; GPNMB: glycoprotein non-metastatic melanoma protein B; NC: negative control; SCI: spinal cord injury.
