TABLE 3.

Summary of in vivo interactions between RB, AP-2, and LT

IPa	Protein detected in coimmunoprecipitates	Coimmunoprecipitation result in:
		Immortalized epithelial cells		LT-transformed cells
		MDCK	HaCat	MDCK(LT)b	MDCK(LTm)c
RB	AP-2	NDd	+	+	ND
	LT			+	−
AP-2	RB	+	+	+	+
	LT			+	−
LT	RB			+	−
	AP-2			+	−

^aAll αAP-2 immunoprecipitations (IP) were done with two or three distinct αAP-2 except with MDCK(LT^m) lysates. Three different αAP-2 were used to identify AP-2 in the LT immunoprecipitations. Two αRB were always used to reveal RB except for the MDCK(LT^m) cells. 

^bMDCK(1-6) cells transformed by wild-type LT. 

^cMDCK(2a5) cells transformed by an LT mutant unable to bind RB. 

^dND, not determined.