Table 2.

Table summarizing the in vitro studies evaluated in this review article.

In vitro studies
Year	Author	Cannabinoid type	Findings
2013	Ramot et al. 21	ACEA and AM251	Keratin 6, a protein which regulates keratinocyte migration, is altered by CB1 agonists and antagonists
2015	Styrczewska et al. 22	Flax fiber extract: CBD, phytosterol, and unsaturated fatty acids	Flax fiber extract showed improved wound healing, decreased inflammation, and promotion of the extracellular matrix after scratch test assays on human skin cells
2017	Bort et al. 23	JWH015	CB2 agonist reduced proinflammatory markers, increased anti‐inflammatory markers, and led to faster scratch gap closure
2018	Ruhl et al. 24	CBD	Multipotent mesenchymal stromal cells treated with CBD showed reduced oxidative stress
2019	Sangiovanni et al. 25	CBD and cannabis sativa extract	Skin cells treated with these compounds showed decreased proinflammatory cytokine release and downregulation of genes which were overexpressed with TNF‐α treatment
2020	Ruhl et al. 26	WIN55,212‐2 and JWH133	Multipotent mesenchymal stromal cells treated with a nonspecific CB receptor ligand and a CB2 agonist had varying effects on their differentiation which, in turn, may affect wound healing
2020	Correia‐Sa et al. 27	N/A	Obtained skin samples from patients who underwent body contouring surgery: noted lower levels of anandamide found in patients who developed hypertrophic scars
2021	Ruhl et al. 28	anandamide, 2‐arachidonoyl‐glycerol, and JWH133	Macrophage and mesenchymal stromal cells treated with endocannabinoids showed downregulation of their inflammatory responses
2021	Correia‐Sa et al. 29	ACEA and AM251	Skin samples from abdominoplasty patients treated with CB1 agonist and antagonist noted that the agonist showed increased collagen deposition and reepithelialization
2021	Correia‐Sa et al. 29	JWH133 and AM630	Fibroblast cells treated with a CB2 agonist and antagonist showed varying effects on collagen deposition depending on whether cells were treated with TGF‐β
2021	Atalay et al. 30	CBD	Keratinocytes treated with H2O2 led to a proinflammatory state which was partially attenuated by CBD
2021	Miller et al. 31	THC and CBD	Multiple cells lines treated with THC and CBD showed improved cell migration and specifically porcine primary fibroblasts showed faster wound closure
2021	Antezana et al. 32	Cannabis sativa extract	Collagen hydrogels loaded with silver nanoparticles and C. sativa extract provided a matrix for tissue growth and showed improved biocompatibility compared to similar products without C. sativa extract

Abbreviations: TGF, transforming growth factor; TNF, tumor necrosis factor.