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. 2024 Feb 15;121(8):e2314561121. doi: 10.1073/pnas.2314561121

Fig. 4.

Fig. 4.

Lpcat4-mediated PC(16:0, 18:2) controls Th17/Treg cell balance. (A) Flow cytometric analysis of IL-17A+ in Th17 (Top) and Foxp3+ in iTreg (Below) cells after treatment with solvent control (Ctrl) or PC(16:0, 18:2) (PC, 50 μM) (n = 3). (B) qPCR analysis of Rorc, Il23r, Foxp3, Il10rb, and Gpr83 mRNA expression in Th17 and iTreg cells after stimulation with control or PC (n = 3 to 4). (C) Immunoblot analysis of RORγt and Foxp3 levels in Th17 and iTreg cells after PC and Ctrl treatments. (D) CBA analysis of IL-17 in Th17 and IL-10 in iTreg cells after PC and Ctrl treatment (n = 3 to 4). (E and F) Flow cytometric analysis of IL-17A+ and Foxp3+ in Th17 and iTreg cells from WT and Setd2-CD4 cre mice (n = 3) after PC or Ctrl treatment. (G) CBA analysis of IL-17 in Th17 cell after PC or Ctrl treatment (n = 3 to 4). Data show one of three independent experiments. Results are presented as means ± SD (A, B, and D–G). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, and ns, not significant. PC referred to PC(16:0, 18:2).