FIG. 1.

(A to C) Effects of insulin on the kinase activity of Akt-WT and Akt-AA in CHO cells. CHO cells were infected with either AxCAAkt-WT or AxCAAkt-AA at an MOI of 5 PFU/cell. The cells were incubated for 10 min in the absence or presence of 100 nM insulin, lysed, and subjected either to immunoblot analysis with antibodies to HA (A) or to immunoprecipitation with antibodies to HA (B and C). The immunoprecipitates were assayed for Akt kinase activity, and the radioactivity incorporated into histone 2B was visualized (B) or quantitated (C) with an image analyzer as described in Materials and Methods. Data are representative of three independent experiments. (D) Effects of Akt-AA and Akt-WT on apoptosis in CHO cells. CHO cells were infected with AxCAAkt-AA (left) or AxCAAkt-WT (right) at the indicated MOI (PFU/cell). The cells were deprived of serum for 16 h, and DNA laddering was examined as described in Materials and Methods. Data are representative of two independent experiments.