| Component | Amount (μl) | Final concentration |
|---|---|---|
| Library DNA (Step 14) | 10 | — |
| 10× NEB buffer 2 | 1.3 | 1× |
| 1 mM dATP | 1.3 | 0.1 mM |
| Klenow (3′→5′Exo−) (5,000 U/ml) | 0.3 | 0.12 U/μl |
| Total volume | 12.9 | — |
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