| Component | Amount (μl) | Final concentration |
|---|---|---|
| Library DNA (Step 16) | 12.9 | — |
| 10× T4 DNA ligase buffer | 1.6 | 1× |
| 15 μM Illumina adaptor oligo mix (Reagent setup) | 0.3 | 0.3 μM |
| 400 U/μl T4 DNA ligase | 1 | 25 U/μl |
| Total volume | 15.8 | — |
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