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In vitro effects of NPs on macrophage repolarization. (A) The relative gene expression levels of iNOS, TNF-α, IL-1β, IL-6, CD206, and IL-10 in macrophages were detected by qRT-PCR. The secretion of macrophage-related proteins iNOS (B and C) and CD206 (D and E) was detected by immunofluorescence staining and the corresponding fluorescence quantity. Original magnification, 100×. Scale bar, 400 μm. n = 3. *P < 0.05, **P < 0.01, ***P < 0.001.
