FIG. 2.

Characterization of Sba1 by gel electrophoresis. (A) Western blot analysis of Sba1 in yeast whole-cell extracts using polyclonal anti-Sba1. Lane 1, 50 μg of whole-cell extract from wild-type (WT) cells; lane 2, 50 μg of whole-cell extract from sba1-1 cells containing pGAL-SBA1 (YF256) grown in glucose-containing medium; lane 3, 5 μg of whole-cell extract from sba1-1 cells containing pGAL-SBA1 grown in galactose-containing medium. The arrowhead shows positions of Sba1 (lane 1) and Sba1^His6 (lane 3). The asterisk shows the band at 60 kDa that reacts with anti-Sba1 in cells overexpressing Sba1^His6. (B) Coomassie blue-stained gel of 2 μg of Sba1^His6 (lane 2) after overexpression in E. coli and protein purification. Molecular size markers (in kilodaltons) shown in lane 1.