FIG. 6.

v-Src and AR activities in wild-type and sba1-1 mutant yeast cells. (A) Western blot analysis of phosphotyrosine activity (upper panel) and the level of v-Src protein (lower panel) in wild-type (WT; strain AFY200; lanes 1 and 2) or sba1-1 (strain AFY202; lanes 3 and 4) cells after growth in glucose (lanes 1 and 3) or galactose (lanes 2 and 4) for 6 h. Molecular size markers are shown in kilodaltons at the left. (B) Quantitation of the level of phosphotyrosine in wild-type and sba1-1 cells from the Western blot shown in panel A. (C) lacZ gene expression in wild-type (strain YF267) and sba1-1 cells (strain YF268) after addition of dihydrotestosterone (DHT) as indicated. Cells were assayed for β-galactosidase 2 h after addition of hormone. Data are means of three independent assays.