Figure 3.

Results of miR133a-3p in acute sepsis-induced acute respiratory distress syndrome. A – Results of hematoxylin and eosin (H&E) staining depicting the morphological changes in sepsis-induced lung tissue after 24 h of injury. The negative control miR-133a-3p antagomir exposed sepsis injury group (NC antagomir) exhibited increase in lung edema, hemorrhage in alveoli, infiltration of neutrophils and damaged epithelial/endothelial cell structure compared to the sham operated group. miR-133a-3p antagomir exposed sepsis animals (miR-133a-3p antagomir) exhibited significant attenuation in morphology of lung tissues. Scale bar, 50 μm. B – ELISA study exhibiting the effect of miR-133a-3p antagomir on levels of TNF-α in sepsis-induced lung injury after 24 h of treatment. C – Activity of MPO in sepsis-induced lung injury 24 h after miR-133a-3p antagomir treatment in every treatment group was recorded. D – Levels of Evan′s blue dye evaluated in each group and treatment of miR-133a-3p antagomir caused a significant reversal and elevated the levels of Evan′s blue dye mediated by sepsis injury after 24 h of treatment. E – Western blot analysis exhibiting the effect of miR-133a-3p on expression levels of protein, i.e. cleaved caspase-3, Bcl-2 and Bax in sepsis injury lung tissues after 24 h of treatment. F – Quantitative analysis results for relative expression of proteins C-caspase, Bcl-2 and Bax

Data are presented as means ± SE (n = 6); ^**p < 0.01 compared to sham operated group.