Figure 6.

Effects of SIRT1 inhibitor EX-527 on the protective effect of miR-133a-3p antagomir in sepsis-induced lung injury. A – H&E demonstrating the changes in morphology of the lung tissue induced by sepsis injury after 24 h of injury. The negative control miR-133a-3p antagomir sepsis injury group (NC-antagomir) demonstrated increase in lung edema, hemorrhage of alveoli, infiltration of neutrophils and damaged epithelial/endothelial cell structure compared to the sham operated group. The miR-133a-3p antagomir sepsis injury group (miR-133a-3p antagomir) demonstrated substantial attenuation on morphology of lung tissues, whereas the SIRT1 inhibitor EX-527 (miR-133a-3p antagomir + EX-527) substantially inverted this improvement. (Scale – 50 μm) B – ELISA study demonstrated the effects of EX-527 treatment on the expression of TNF-α in sepsis-induced lung injury. C – Activities of MPO in sepsis injured lung tissues after 24 h of treatment of miR-133a-3p antagomir in presence or absence of without EX-527 in every group. D – Levels of Evan′s blue dye were studied in each of the three; the results suggested that EX-527 reversed the decrease in levels of Evan′s blue dye mediated by miR-133a-3p antagomir. E – Western blot analysis for demonstrating the effect of EX-527 on protein levels of cleaved caspase-3, Bax, and Bcl-2 in sepsis injured lung tissues after 24 h of treatment

The results are means ± SE (n = 6). ^**P < 0.01.