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. 1998 Jul;18(7):3782–3787. doi: 10.1128/mcb.18.7.3782

FIG. 4.

FIG. 4

The HU replication checkpoint decreases the rate of Cdc2 tyrosine dephosphorylation. A synchronous early-S-phase culture of wee1-50 mik1Δ cells was prepared by elutriation of cells pretreated with HU. For half of the culture, the HU was removed and the cells were given 80 min to complete S phase. Both halves of the culture were shifted to 35°C at 0 min to inactivate Wee1. Cell cycle progression and tyrosine phosphorylation of Cdc2 were monitored as in the experiment for which results are shown in Fig. 1.