Figure 6.

S-amlodipine-induced hepatic inflammation in rats is mediated by disordered intestinal microbiota and their LPS. (a) Representative pictures of liver sections stained with H&E, Oil Red O, or Sirius Red were obtained. Arrows indicate regions of lesions. (b) The histology score of the liver was evaluated (b), and the quantitation of the Oil-Red-O area (c) and fibrosis area was performed (d). N = 3 per group. Scale bars, 50 μm. (e-f) the serum levels of TNF-α (e) and IL-6 (f) were measured by ELISA. N = 5 per group. (g) Hepatic TNF-α level was determined. N = 5 per group. (h) The mRNA expression of inflammatory genes in the liver was detected by RT-qPCR. N = 5 per group. (i) The mRNA expression of Nfκb1, chemokine CCL2, and TLR4 genes in the liver was detected by RT-qPCR. N = 5 per group. (j-k) Representative images of western blot analysis on liver tissues were obtained (j), and the protein expression of TNF-α and TLR4 was quantified (k). *p < 0.05, **p < 0.01. ns, no significant difference.