Fig. 6. Screening combinational hyperthermia and chemotherapy in 3D in vitro tumor models: (A1) live/dead confocal images of control untreated C6 spheroids, and spheroids treated with AuN, AuNDIP, and Mix (DOX-ICG and B-anti G)), with and without laser exposure (scale bar = 100 μm). Reprinted from ref. 287, Copyright (2022), with permission from Elsevier. (B1) Confocal images of A2780 cells and spheroids: (a) and (b) non-treated with aAuYs under laser irradiation; (c) and (d) treated with aAuYS without laser irradiation, and (e) and (f) treated with aAuYS under laser irradiation (scale bars in the upper and lower panels represent 6 and 20 μm, respectively), and (B2) infrared thermal images of a mice treated with DOX and aAuYS submitted to NIR laser, Reprinted with permission from ref. 288. Copyright 2021 American Chemical Society; (C1) scheme representing the performed procedure of hBM-MSCs NPs delivery in 3D spheroids followed by laser irradiation; (C2) heat map enhancing the influence of the number of MSCs loaded with PDA-ICG-DOX NPs in 3D spheroids viability, 3 days after NIR laser treatment (irradiation (NIR +), and without irradiation (NIR −)). Reprinted with permission from ref. 174, Copyright (2021), with permission from Elsevier; (D1) scheme representing the incorporation of macrophage loaded with SPIONs into cancer cells and its effect when submitted to an AMF; (D2) confocal microscopy showing the integration of J774a.1 macrophages (red) in K-EC spheroids (green) (scale bar = 50 μm); and (D3) metabolic activity of re-cultured spheroids containing T-SPION loaded macrophages with or without AMF application after 48 h of treatment. Reprinted with permission from ref. 292, Copyright (2019), with permission from Elsevier.