Table 1.
Single actin filament dynamics in wild-type (ARP2) and arp2-1 mutant with or without CK-666
| Genotype and treatment | ||||
|---|---|---|---|---|
| Stochastic dynamic parameters |
ARP2 w/ DMSO |
arp2-1 w/ DMSO |
ARP2 w/ CK-666 |
arp2-1 w/ CK-666 |
| Elongation rate; µm/s | 1.79 ± 0.05a | 1.90 ± 0.07b | 1.89 ± 0.07b | 1.90 ± 0.07b |
| Max. filament length; µm | 16.6 ± 0.3a | 17.4 ± 0.2b | 17.2 ± 0.2b | 17.8 ± 0.3b |
| Max. filament lifetime; s | 18.7 ± 0.4a | 22.6 ± 0.3b | 22.0 ± 0.3b | 22.9 ± 0.3b |
| Severing frequency; breaks/mm/s | 8.20 ± 0.11a | 7.93 ± 0.12a | 7.60 ± 0.19b | 8.18 ± 0.20a |
Measurements were taken from epidermal cells in the elongating apical region of 5-d-old dark-grown hypocotyls. Values represent mean ± Se. n ≥ 100 filaments from 2 biological repeats conducted with independent plant materials (for 1 biological repeat, 5 filaments were counted in 1 hypocotyl from at least 10 hypocotyls per treatment/genotype). By 2-way ANOVA with Tukey's post hoc test, letters a and b denote genotypes or treatments that show statistically significant differences with other genotypes or treatments, P < 0.05.