Table 1.
Statistics for data collection, refinement and validation
| CaV1.2(ΔC)/CaVβ3/CaVα2δ-1:GBP | ||
|---|---|---|
| Data collection and processing | ||
| Magnification | 105,000 | |
| Voltage (kV) | 300 | |
| Electron dose (e−/Å2) | 46 | |
| Defocus range (μm) | −0.9~−1.7 | |
| Pixel size (Å) | 0.835 | |
| Symmetry | C1 | |
| Initial particle images (no.) | 9,703,873 | |
| CaV1.2(ΔC)/CaVβ3/CaVα2δ-1:GBP (PDB 8FD7; EMD-29004) | EMC:CaV1.2(ΔC)/CaVβ3 (EMD-29006) | |
| Final particle images (no.) | 259,107 | 383,185 |
| Map resolution (Å) | 3.1 | 3.1 |
| FSC threshold | 0.143 | 0.143 |
| Map resolution range (Å) | ~2.4–8.0 | ~2.4–8.0 |
| Refinement | ||
| Initial model used (PDB code) | 8EOG | |
| Model resolution (Å) | 3.3 | |
| FSC threshold | 0.5 | |
| Map sharpening B factor (Å2) | −20 | |
| Model composition | ||
| Nonhydrogen atoms | 19,701 | |
| Protein residues | 2,416 | |
| Ligands | 20 | |
| B factors (Å2) | ||
| Protein | 118.63 | |
| Ligand | 42.23 | |
| r.m.s. deviations | ||
| Bond lengths (Å) | 0.002 | |
| Bond angles (°) | 0.479 | |
| Validation | ||
| MolProbity score | 2.16 | |
| Clashscore | 7.54 | |
| Rotamer outliers (%) | 2.76 | |
| Ramachandran plot | ||
| Favored (%) | 93.78 | |
| Allowed (%) | 6.10 | |
| Outliers (%) | 0.13 |