FIGURE 1.

Both Canonical ECS Receptors Are Present In The Spleen Of Mice, Rats, and NHP. Relative expression of cnr1 and cnr2 was determined using qPCR from the colon, heart, kidney, liver, MLN, spleen, and visceral fat from mice, rats, and NHP. (a) In mice, we detected cnr1 mRNA in the colon, kidney (4/5 mice), liver (2/5 mice), spleen, and visceral fat, having the highest levels in the latter. Significant differences were found in the levels of expression between the colon and the heart, as well as among the visceral fat and the levels of expression in the heart and the liver. (b) Cnr1 was detected in partial samples of the rat model colon (4/6 rats), heart (1/6 rats), kidney, and spleen (1/6 rats), having statistically higher levels of expression in the kidney when compared to the heart, liver, MLN, and spleen. (c) In NHP, cnr1 mRNA was detected in the spleen (2/3 rhesus) and visceral fat at comparable levels. (d) Cnr2 was detected only in the spleen and visceral fat (4/5 mice), at comparable levels. The spleen had statistically significantly higher levels as compared to the colon, heart, kidney, and liver. (e) Cnr2 was detected in the colon (2/4 rats), heart (1/6 rats), MLN (4/6 rats), and spleen of rats. (f) In NHP, cnr2 mRNA was detected in the liver (1/4 rhesus), MLN, and spleen. Detection levels were significantly higher in the spleen when compared to the heart, kidney, and liver, but with significant differences when compared to the liver (1/4 rhesus), kidney, and heart. Data are represented as the mean ± SD.