FIGURE 2.

Peroxisome Proliferator Activated Receptors, Ppara and Pparg, Are Generally Well Conserved In All Organs Of Mice, Rats and NHP. Relative expression of ppara and pparg was determined using qPCR from the colon, heart, kidney, liver, MLN, spleen, and visceral fat from mice, rats, and NHP. (a) Ppara mRNA was detected in all the organs tested in mice, except for the spleen. It had significantly higher levels in the kidney when compared with the colon and the spleen. (b) In rats, ppara mRNA was detected in the colon (4/6 rats), heart, liver, kidney, MLN (5/6 rats), and spleen (4/6 rats). Ppara in the rat model was significantly higher in the heart, kidney, and liver, when compared to the colon, MLN, and spleen. (c) Ppara was detected in all organs tested in the periphery of the NHP model at comparable levels for all the evaluated organs. (d) Mouse pparg was similar to ppara, being detected in the colon (3/5 mice), heart (4/5 mice), kidney, liver (4/5 mice), and the visceral fat. Detection of this gene was higher in visceral fat when compared to the colon, kidney, liver, and spleen. (e) Pparg was detected in all the organs available for the rat model with no statistical significance among any organ. (f) Pparg in the NHP model was also detected in all available organs for the NHP model, having only partial detection in the heart (1/2 rhesus) and the kidney (3/4 rhesus). Interestingly, pparg was significantly higher in the visceral fat when compared with all other tissues. It is worth mentioning that neither of these genes were detected in the spleen of mice, contrary to the other animal models. Data are graphed as the mean ± SD.