TABLE 3.
SH3 and actin-binding domains are required for HS1 to suppress Ras-induced malignancy
| v-Ha-Ras-transformed NIH 3T3 cellsa | Colonies formed in soft agar/103 cellsb |
|---|---|
| Vector alone | 648 ± 122 |
| HS1 (full length) | 148 ± 30 |
| Vector alone | 600 ± 43 |
| HS1 (SH3 negative) | 565 ± 103 |
| Vector alone | 865 ± 143 |
| HS1 (ACT negative) | 645 ± 97 |
| Vector alone | 728 ± 19 |
| HS1-EMS1 chimera (ACT negative) | 678 ± 25 |
a
HS1 (SH3 negative), lacking SH3 domain; HS1 (ACT negative), lacking the actin-binding domain; HS1-EMS1, non-actin-binding chimera.
b
Each value is the average of data from at least four individual clones in duplicate assays ± standard deviation.