FIG. 2.

P130 consists of two glycoproteins, one a SIRP family member and the other PIR-B/p91A. (A) P130 consists of two distinct glycoproteins in primary BMM and cell lines derived from them. SHP-1 immunoprecipitates (IP) from randomly growing normal primary BMM (left panel) or N and meV cell lines (right panel) were treated with endo F (+) or left untreated (−). Deglycosylated proteins were analyzed by SDS-PAGE (8% gel) and anti-pTyr immunoblotting. The migration of molecular size standards is indicated in kilodaltons at the left. (B) Identification of the two glycoproteins as an SHPS-1-related protein and PIR-B/p91A. Preimmune (PI), anti-SHP-1, and anti-SHPS-1 immunoprecipitates from the meV cell line were treated with endo F (+) or left untreated (−) and then immunoblotted with anti-SHPS-1 (left panel) and anti-pTyr (middle panel) antisera. The anti-pTyr blot was stripped and reprobed with anti-PIR-B antisera (right panel).