FIG. 5.

BIT and PIR-B tyrosine phosphorylation in N, meV, and me/me primary BMM. (A) BIT and PIR-B are hyperphosphorylated in primary BMM from me^ν/me^ν mice. The relative levels of tyrosine phosphorylation of BIT and PIR-B in anti-SHP-1, anti-SHPS-1 (which recognizes murine BIT), and anti-PIR-B immunoprecipitates (IP) from N and meV primary BMM were compared by anti-pTyr immunoblotting (upper panels) and by reprobing and immunoblotting for protein levels (lower panels). The migration of molecular size standards is indicated in kilodaltons at the left. (B) BIT and PIR-B are hypophosphorylated in primary BMM from me^ν/me^ν mice. As in panel A, relative tyrosine phosphorylation levels in anti-SHPS-1 and anti-PIR-B immunoprecipitates from normal and me^ν/me^ν primary BMM were compared by anti-pTyr immunoblotting (upper panels) and immunoblotting for protein levels (lower panels). In addition, immunoprecipitates were endo F-treated to facilitate the visualization of BIT and PIR-B (right-hand panels). The migration of molecular size standards is indicated in kilodaltons at the left.