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. 2024 Feb 4;27(2):324–342. doi: 10.1007/s10120-023-01461-7

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — TLRs mRNA expression in (A) wild-type GES-1 cells (control), GES-1 cells persistently infected with H. pylori for 1 generation (Hp-1) and 30 generations (Hp-30) or in (B) control cells, GES-1 cells co-cultured with H. pylori lysate for 1 generation (lysate-1) and 30 generations (lysate-30). TLR6 mRNA expression in (C) control cells, Hp-1 cells, Hp-10 cells, Hp-20 cells and Hp-30 cells or in (D) control cells, lysate-1 cells, lysate-10 cells, lysate-20 cells and lysate-30 cells. TLR6 protein expression (E) and TLR6 immunofluorescent staining (G) in control cells, Hp-1 cells and Hp-30 cells. TLR6 protein expression (F) and TLR6 immunofluorescent staining (H) in control cells, lysate-1 cells and lysate-30 cells. All experiment were performed in triplicate and all data represents means ± SEM. ***P < 0.001, **P < 0.01, *P < 0.05. Scale bars, 10 µm (G, H)

Fig. 1 — TLRs mRNA expression in (A) wild-type GES-1 cells (control), GES-1 cells persistently infected with H. pylori for 1 generation (Hp-1) and 30 generations (Hp-30) or in (B) control cells, GES-1 cells co-cultured with H. pylori lysate for 1 generation (lysate-1) and 30 generations (lysate-30). TLR6 mRNA expression in (C) control cells, Hp-1 cells, Hp-10 cells, Hp-20 cells and Hp-30 cells or in (D) control cells, lysate-1 cells, lysate-10 cells, lysate-20 cells and lysate-30 cells. TLR6 protein expression (E) and TLR6 immunofluorescent staining (G) in control cells, Hp-1 cells and Hp-30 cells. TLR6 protein expression (F) and TLR6 immunofluorescent staining (H) in control cells, lysate-1 cells and lysate-30 cells. All experiment were performed in triplicate and all data represents means ± SEM. ***P < 0.001, **P < 0.01, *P < 0.05. Scale bars, 10 µm (G, H)