FIG. 2.

A mutant STAT5B harboring mutations homologous to those in STAT5A1*6 induces IL-3-independent growth of Ba/F3 cells. Proliferation after transduction of the sequences for the wild-type STAT5A (5A WT) or STAT5B (5B WT) or mutated STAT5A (5A 1*6) STAT5B (5B 1*6) was examined with bulk populations of Ba/F3 cells after retrovirus-mediated transduction with these constructs. The infection efficiency in this experiment was 30 to 50%. Proliferation of parental Ba/F3 cells without IL-3 is also shown.