Fig. 8. TYA-018 regulates the acetylation of proteins associated with myofilaments, mitochondrial metabolic enzymes, and calcium regulation in HFpEF mice.

a Schematic overview illustrating acetylome workflow. b Pie chart shows STRING analysis of enriched proteins with altered acetylation in HFpEF mouse hearts after TYA-018 treatment. c Heatmap of altered lysine acetylation at sites of selected proteins after STRING analysis. Red represents relative increases in the lysine acetylation, and blue represents relative decreases in lysine acetylation. Proteins with their respective acetylation sites are listed on the left side of each row (n = 3 animals per group). d Representative western blot of tropomyosin (Tpm), troponin I (TnnI), and myosin heavy chain 6 (Myh6) after immunoprecipitation with anti-lysine acetylation antibody in heart samples. Immunoglobulin G (IgG) pulldown was used as a control.