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. 2024 Jan 2;25(2):876–901. doi: 10.1038/s44319-023-00044-y

Figure EV1. FANCJ, but not DDX11, directly interacts with AND-1 6xHis-SepB.

Figure EV1

(A) Schematic representation of the polypeptide chain of human FANCJ. Conserved sequence motifs and domains are indicated with the same abbreviations and colors used in Fig. 1A. In the insert, alignment of human FANCJ and budding yeast Chl1 CIP box sequence is reported with corresponding regions of other SF2 Fe–S human DNA helicases. Abbreviations used are HUMAN, Homo sapiens and YEAST, Saccharomyces cerevisiae. The KALIGN tool (version 3.3.1) was used. Highly conserved amino-acid residues are highlighted in green. (B) Co-pull-down experiments of Flag-tagged FANCJ WT or AALA mutant and AND-1 6xHis-SepB using anti-Flag agarose beads. Pulled-down samples were analyzed by Western blot with an anti-Flag peroxidase-conjugated mouse monoclonal antibody (Sigma-Aldrich cat. A8592) and anti-poly-Histidine peroxidase-conjugated mouse monoclonal antibody (Sigma-Aldrich cat. A7058). Schematic of the recombinant proteins used in the pulled-down experiments is shown.