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. 2024 Jan 8;25(2):832–852. doi: 10.1038/s44319-023-00033-1

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. Creative Commons Public Domain Dedication waiver http://creativecommons.org/publicdomain/zero/1.0/ applies to the data associated with this article, unless otherwise stated in a credit line to the data, but does not extend to the graphical or creative elements of illustrations, charts, or figures. This waiver removes legal barriers to the re-use and mining of research data. According to standard scholarly practice, it is recommended to provide appropriate citation and attribution whenever technically possible.

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Figure EV5 — (A,B) Migration and invasion was analysed by Transwell assays in shScr, shBRD4-L and shBRD4-S by transfecting them with siScr, siITGA4, siITGA5 or siITGA4 and 5. The inserts were stained with crystal violet. Images are representative of at least three biological replicates. Scale bar: 100 μm. Scatter plots indicating the percentage of migration and invasion is shown. Error bars correspond to the average ± SEM (n = 5 biological replicates with 2 technical replicates shown). Two-tailed non-parametric unpaired t test was performed for statistical analysis. ***p ≤ 0.001, ****p ≤ 0.0001. (C) Western blot analysis showing transient ITGA4 and ITGA5 knockdown efficiency in shScr, shBRD4-L and shBRD4-S cells as indicated. β-Actin was used as the loading control. Images are representative of at least three biological replicates. (D) Western blot analysis showing ZEB2 and c-MYC expression in shScr, shBRD4-L and shBRD4-S cells as indicated. β-Actin was used as the loading control. Images are representative of at least two biological replicates. (E) Graphical model summarizing the function of the BRD4 isoforms in ERMS. Source data are available online for this figure.