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. 2024 Feb 26;15(2):174. doi: 10.1038/s41419-024-06554-4

Fig. 5. miR-184-repression of Wnt/β-catenin contributes to SCC phenotype.

Fig. 5

A–C The indicated samples of murine wild type (WT) or knockout (KO) tumors or human squamous cell carcinoma C12C20 line that were infected with control (Ctl) or anti-miR-184 antagonist (AM184) were lysed and subjected to Western blot analysis of β-Catenin (β-Cat) or GAPDH as a loading control. Quantified data is shown on the right. Immunofluorescent (D) and its quantification (E) and immunohistochemistry (F) staining showing increased β-Cat staining in tumors of AM184 infected SCC cells. E Quantification of the nuclear signal (arrows in D) was performed by Image J software in 7 different fields in each of the 3 biological replicates. Each dot represents an average of a single field. G, H Colony formation assay performed using the indicated SCC cell line (A431 or C12C20) that were infected with Ctl or AM184 and treated with vehicle or with the β-Catenin/TCF4 inhibitor PKF118-310 as detailed in Methods. Representative image shown in (G) and quantification is shown in (H). Data in represents 3 biological replicates. Scale bars were 20 μm. Statistical significance was assessed by t test (*p < 0.05).