Table 1.
Yeast strains used in this study.
| Name | Genotype | Background | Source |
|---|---|---|---|
| BY4741 | his3∆1 leu2∆0 met15∆0 ura3∆0; Mat a | BY474x | Euroscarf |
| yABH1 | pho8::pho8∆60:His ATG13wt:Ura; Mat alpha | BY474x | This study |
| yABH2 | pho8::pho8∆60:His ATG13CD:Ura; Mat alpha | BY474x | This study |
| yABH3 | pho8::pho8∆60:His ATG1344D:Ura; Mat alpha | BY474x | This study |
| yABH6 | pho8::pho8∆60:His ATG13NA:Ura; Mat alpha | BY474x | This study |
| yABH7 | pho8::pho8∆60:His ATG13ND:Ura; Mat alpha | BY474x | This study |
| yABH8 | pho8::pho8∆60:His ATG13CA:Ura; Mat alpha | BY474x | This study |
| yABH11 | ATG13wt:Ura; Mat a | BY474x | This study |
| yABH17 | pho8::pho8∆60:His ATG1344A:Ura; Mat alpha | BY474x | This study |
| yABH25 | ATG1344D:Ura; Mat a | BY474x | This study |
| yABH26 | ATG1344A:Ura; Mat a | BY474x | This study |
| yABH59 | pho8::pho8∆60:His ATG138SA:Ura; Mat alpha | BY474x | This study |
| yABH60 | pho8::pho8∆60:His ATG138SD:Ura; Mat alpha | BY474x | This study |
| yABH62 | pho8::pho8∆60:His ATG13MA:Ura; Mat alpha | BY474x | This study |
| yABH63 | pho8::pho8∆60:His ATG13MD:Ura; Mat alpha | BY474x | This study |
| yABH73 | pho8::pho8∆60:His atg1::Kan atg11::Hyg atg13::Kan; Mat a | BY474x | This study |
| yABH76 | pho8::pho8∆60:His ATG13379D:Ura; Mat alpha | BY474x | This study |
| yABH78 | pho8::pho8∆60:His ATG13428D429D:Ura; Mat alpha | BY474x | This study |
| yABH79 | pho8::pho8∆60:His ATG13429D:Ura; Mat alpha | BY474x | This study |
| yABH80 | pho8::pho8∆60:His ATG13428A429A:Ura; Mat alpha | BY474x | This study |
| yABH81 | pho8::pho8∆60:His atg11::Nat ATG13379D:Ura; Mat a | BY474x | This study |
| yABH86 | pho8::pho8∆60:His atg11::Nat ATG13wt:Ura; Mat a | BY474x | This study |
| yABH90 | atg8::His atg13::Kan atg19::Hyg; Mat a | BY474x | This study |
| yABH91 | atg8::Kan atg13::Nat atg17::His atg19::Kan; Mat a | BY474x | This study |
| yCK416 | atg11:Kan; Mat a | BY474x | (Pfaffenwimmer et al, 2014) |
| yCK418 | atg13::Kan; Mat a | BY474x | This study |
| yDH11 | pho8::pho8∆60:His; Mat alpha | BY474x | This study |
| yDH516 | pho8::pho8∆60:His atg13::Kan atg1::Kan; Mat alpha | BY474x | This study |
| yDP441 | atg1::Kan atg13::Nat atg19::Kan; Mat a | BY474x | This study |
| yDP445 | atg13::Nat atg19::Kan; Mat a | BY474x | This study |
| yDP451 | atg19::Hyg; Mat a | BY474x | This study |
| yDP454 | atg1::Kan atg11::Hyg atg13::Nat atg19::Kan; Mat a | BY474x | This study |
| yRT15 | Atg1-stag(protA-speptide):His atg13::Nat atg17-13myc:Kan Vac8-HA:Kan; Mat a | BY474x | This study |
| yRT70 | atg1::Kan atg11::Hyg atg13::Kan; Mat alpha | BY474x | (Torggler et al, 2016) |
| yRT105 | atg1::His atg13::Nat; Mat alpha | BY474x | This study |
| yRT199 | pho8::pho8∆60:His atg1::Kan; Mat a | BY474x | This study |
| yRT238 | atg13::Kan pep4∆::Nat; Mat a | BY474x | This study |
| ySS15 | pho8::pho8∆60:His atg13::Kan; Mat alpha | BY474x | This study |
| ySS22 | pho8::pho8∆60:His atg13::Kan atg11::Nat; Mat a | BY474x | This study |
| ySS24 | pho8::pho8∆60:His atg13::Kan atg17::His; Mat a | BY474x | This study |
| ySS31 | pho8::pho8∆60:His atg13::Kan atg19::Kan; Mat alpha | BY474x | This study |
| yTP210 | Atg1-stag(protA-speptide):His atg13::Nat; Mat alpha | BY474x | (Torggler et al, 2016) |
Description: Atg13 constructs were genomically integrated by homologous recombination into the atg13::Kan locus in the following BY474x strains:
pho8::pho8∆60:His atg13::Kan (ySS15) or atg13::Kan (yCK418).