Table 2.
Centromeric plasmids used in this study.
| Name | Characteristics | Promoter | Terminator | Source |
|---|---|---|---|---|
| pRS315 | CEN, LEU2 | – | – | (Sikorski and Hieter, 1989) |
| pRS316 | CEN, URA3 | – | – | (Sikorski and Hieter, 1989) |
| pABH15 | Atg1-13CD; pRS415 | Atg1 | CYC1 | This study |
| pABH41 | Atg138SD; pRS316 | Atg13 | Atg13 | This study |
| pABH45 | Atg1-13MD; pRS415 | Atg1 | CYC1 | This study |
| pABH46 | Atg1-1344D; pRS415 | Atg1 | CYC1 | This study |
| pABH48 | Atg1344A-GFP; pRS415 | Atg13 | CYC1 | This study |
| pABH49 | Atg1344D-GFP; pRS415 | Atg13 | CYC1 | This study |
| pABH53 | Atg13MD-GFP; pRS415 | Atg13 | CYC1 | This study |
| pABH55 | Atg13CD-GFP; pRS415 | Atg13 | CYC1 | This study |
| pABH65 | Atg1-13CD-GFP; pRS415 | Atg1 | CYC1 | This study |
| pABH66 | Atg1-13wt-GFP; pRS415 | Atg1 | CYC1 | This study |
| pABH71 | Atg137SD1A; pRS316 | Atg13 | Atg13 | This study |
| pCK111 | Atg1; pRS315 | Atg1 | Atg1 | (Kijanska et al, 2010) |
| pCK582 | Atg13wt-GFP; pRS415 | Atg13 | CYC1 | (Torggler et al, 2016) |
| pCK789 | Atg13; pRS316 | Atg13 | Atg13 | This study |
| pDP227 | Atg1-GFP; pRS415 | Atg1 | CYC1 | This study |
| pMS98 | Atg1-13FV; pRS415 | Atg1 | CYC1 | This study |
| pRT09 | Atg1346A; pRS316 | Atg13 | Atg13 | This study |
| pRT10 | Atg1346D; pRS316 | Atg13 | Atg13 | This study |
| pRT15 | Atg1344A; pRS316 | Atg13 | Atg13 | This study |
| pRT16 | Atg1344D; pRS316 | Atg13 | Atg13 | This study |
| pRT67 | Atg1-13wt; pRS415 | Atg1 | CYC1 | This study |