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. 2024 Jan 12;43(3):462–480. doi: 10.1038/s44318-023-00005-0

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© This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2024

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(A) Chemical structures of inositol phosphates incubated with TvIPK in the experiments described in this figure; phosphate numbering follows published guidelines (Murthy, 2006; Pramanik et al, 2020). See Appendix Fig. S1 for structures of other inositol phosphates used in this study. (B–E) Representative (1 of 3 independent experiments) HPLC data following incubations containing [γ-³³P]-ATP, either with TvIPK (blue traces) or without enzyme (red trace), plus 50 µM of (B) myo-IP₆ or (C) myo-(1OH)IP₅ or (D) myo-(2OH)IP₅ or (E) myo-(3OH)IP₅. (F) PP-IP product accumulation from assays performed either in the absence (open bars) or presence of HsDIPP1 (closed bars) upon (i.e., apparent kinase activity) after incubation of TvIPK with 1 mM ATP and 200 µM of the indicated myo- or scyllo-IPs; (G) Substrate saturation plot for TvIPK incubated with 1 mM ATP and the indicated concentrations of myo-[³H]IP₆; activities were analyzed by HPLC. (H) Substrate saturation plot for TvIPK incubated with 2 mM myo-IP₆ and the indicated concentrations of [γ-³³P]-ATP; activities were analyzed by HPLC. (F–H) show each data point obtained from either 3 or 6 independent experiments (some data points are superimposed); standard errors are also indicated by vertical bars. Source data are available online for this figure.