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. 2024 Jan 12;43(3):462–480. doi: 10.1038/s44318-023-00005-0

Table 2.

Single site mutagenesis of TvIPK: impact on kinase activity against myo-IP6.

Activity (nmol/mg protein/min)
WT 246.6 ± 5
R54A 50.6 ± 5.4
G57A 4.6 ± 0.4
R58A 11.6 ± 0.6
K76A 22.4 ± 0.9
K128A 7.3 ± 0.7
R132A 0.6 ± 0.1
K133A 32.6 ± 0.7
R134A 213.5 ± 1
K135A 65.7 ± 1.5
R145A 58.4 ± 1
R234A 2.7 ± 0.2

Assays were performed with 50 µM [γ-33P]-ATP and 50 µM of each substrate and were analyzed by HPLC. Data represent means and standard errors from 3 independent experiments. Student’s t test was used to determine statistical significance of each mutant vs wild type enzyme. For R134A, p = 0.03. For the other mutants, p < 0.002.