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. 2023 Dec 14;25(1):378–403. doi: 10.1038/s44319-023-00012-6

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. Creative Commons Public Domain Dedication waiver http://creativecommons.org/publicdomain/zero/1.0/ applies to the data associated with this article, unless otherwise stated in a credit line to the data, but does not extend to the graphical or creative elements of illustrations, charts, or figures. This waiver removes legal barriers to the re-use and mining of research data. According to standard scholarly practice, it is recommended to provide appropriate citation and attribution whenever technically possible.

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Figure EV1 — (A) Heatmap showing the tissue specificity of 80 mitochondrial proteins that were highly expressed in liver tissue. (B, C) H&E staining and Oil Red O staining of liver sections of the mice fed a high-fat diet (HFD) for the indicated times. Scale bars, 50 μm. (D, E) Western blots probed with antibodies against NME4 and Actin in the livers of mice fed a normal diet or a high-fat diet (HFD) for the 2 weeks and 6 weeks. (F) Relative mRNA levels of NME4 in the livers of the mice fed a normal diet or HFD for 2 weeks was measured by RT‒qPCR. Biological replicates, n = 3. (G, H) Western blots probed with antibodies against NME4, UCP1 and Actin in the subcutaneous white adipose tissue (sWAT), epididymal white adipose tissue (eWAT) and brown adipose tissue (BAT) of mice fed a normal diet or a high-fat diet (HFD) for the 6 weeks. (I–K) Western blots probed with antibodies against NME4, UCP1 and Actin in the sWAT, eWAT, BAT and liver of mice fed a normal diet or a high-fat diet (HFD) for the 24 weeks. Data information: (F) data are presented as mean ± SEM. **P values ≤ 0.01 (Student’s t test, unpaired). w, week; HFD high-fat diet, sWAT subcutaneous white adipose tissue, eWAT epididymal white adipose tissue, BAT brown adipose tissue.