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. 2023 Dec 14;25(1):45–67. doi: 10.1038/s44319-023-00009-1

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. Creative Commons Public Domain Dedication waiver http://creativecommons.org/publicdomain/zero/1.0/ applies to the data associated with this article, unless otherwise stated in a credit line to the data, but does not extend to the graphical or creative elements of illustrations, charts, or figures. This waiver removes legal barriers to the re-use and mining of research data. According to standard scholarly practice, it is recommended to provide appropriate citation and attribution whenever technically possible.

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Figure EV2 — (A) Representatives images of MFN2 KO MEFs control or transfected with MFN2-K109A-FLAG, MFN2-IYFFT-MYC, MFN2-ACTA-V5, WT MFN2-MYC, or co-transfected with MFN2-IYFFT-MYC and MFN2-ACTA-V5. Mitochondria were stained with αTOMM20 antibody. Scale bar 10 µm; inset scale bar 5 µm. (B) Mitochondrial aspect ratio analysis (at least 25 control and 25 transfected cells were analyzed per condition). One-way ANOVA statistical analysis and SEM are shown. (C) Representative images of WT and MTCH2 KO cells transfected with the ER marker Sec61b-GFP and stained with αTOMM20 antibody. Arrowheads indicate areas of close proximity between ER and mitochondria. Scale bar 10 µm; inset scale bar 2 µm. (D) Representatives images of WT and MTCH2 KO MEFs co-transfected with the ER-mitochondria linker construct AKAP-mtagBFP2-UBC6 and the ER marker Sec61b-GFP. Mitochondria were labeled with MTDR. Scale bar 10 µm; Scale bar inset 5 µm. (E) Mitochondrial aspect ratio analysis. At least 15 control and 15 transfected cells were analyzed per condition. One-way ANOVA statistical analysis and SEM are shown. Data information: (B,E) ns, not significant, ***P < 0.001. Source data are available online for this figure.