FIG. 4.

Preferential binding of TBP to damaged DNA. CDDP-, TDDP-, Dach-, or Dien-treated (100 lesions/plasmid); EtAz-treated (30 lesions/plasmid); or AAF-treated (15 to 20 lesions/plasmid) DNA or untreated (NT) DNA was labelled with [α-³²P]dATP. NT or damaged DNA was then incubated with increasing amounts of TBP and tested for retention on nitrocellulose filters. Graphs represent the percentages of DNA retained on the filters as a function of the amount of TBP. One hundred percent represents the total counts obtained when 1 μl of each DNA probe (input) was spotted onto Whatman paper and simultaneously exposed with the nitrocellulose filter.