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. 1998 Jul;18(7):3907–3914. doi: 10.1128/mcb.18.7.3907

FIG. 5.

FIG. 5

Footprinting of TBP onto DNA containing either a TATA box or CDDP damage. (A) TBP footprint on the TATA box could be competed by adding increasing amounts (10, 20, and 30 ng) of either undamaged (NT) or CDDP-, TDDP-, Dach-, or Dien-damaged DNA (containing 100 lesions per molecule) or TATA box-containing fragment (AdMLP). Lanes 1 and 22, naked DNA; lanes 2 and 21, no DNA competitor. (B) Footprint of TBP onto a 1,2-d(GpG) cisplatin lesion (T) compared with NT DNA. (C and D) Footprint of TBP onto a 1,2-d(GpG) cisplatin lesion (T) compared with a TATA box-containing fragment in the presence or absence of TFIIB as indicated. The TATA box is boxed and the 1,2-d(GpG) cisplatin lesion position is shaded on the left of the figure, and TBP footprint protection is determined upon sequencing.