Figure 2.

H/+ mice exhibit normal early physical development and postnatal behavior

(A) Depiction of mouse developmental stages. Neonatal and young adolescent developmental milestones were evaluated.

(B) Body lengths of +/+ and H/+ mice are identical across the neonatal and early adolescent periods.

(C) Standard mouse developmental and motor behavior benchmarks were achieved at similar time points for both +/+ and H/+ mice.

(D) H/+ mice exhibit normal progression of negative geotaxis (quantified as a geotaxis score).

(E and F) H/+ and +/+ mice succeed in surface righting by P9 as measured by duration to achieve surface righting (E) and surface righting core (F).

(G) H/+ mice show a normal auditory startle reflex and achievement of motor behavioral milestones such as horizontal screen, vertical screen, cliff avoidance, and quadruple walking. See also Table S1. Data shown are mean ± SEM.

Statistical analysis: two-way ANOVA with Šídák’s multiple comparisons test (B and D–F). For (B)–(G): +/+ mice, n = 20; H/+ mice, n = 18.