REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Mouse BD Fc block	BD Biosciences	Cat # 553142; RRID: AB_394656
PE anti-mouse PDL1	Biolegend	Cat # 155404; RRID: AB_2728222
AF700 anti-mouse CD8	BD Biosciences	Cat # 557959; RRID: AB_396959
APC anti-mouse CD45	BD Biosciences	Cat # 559864; RRID: AB_398672
Human TruStain FcX	Biolegend	Cat # 422302; RRID: AB_2818986
PE anti-human PD-L1	Biolegend	Cat # 329706; RRID: AB_940368
Pacific Blue anti-mouse CD40	BioLegend	Cat # 124626; RRID: AB_2561476
BUV395 Rat Anti-Mouse H-2 Class I	BD Biosciences	Cat # 749705; RRID: AB_2873959
PE rat anti-mouse CD86	BD Biosciences	Cat # 553692; RRID:AB_394994
APC rat anti-mouse CD209a	BD Biosciences	Cat # 564928; RRID:AB_2739011
BV421 anti-human CD4	Biolegend	Cat # 317434; RRID: AB_2562134
BUV805 mouse anti-human CD8	BD Biosciences	Cat # 612889; RRID: AB_2833078
FITC Ki-67 monoclonal antibody	eBiosciences	Cat # 11-5698-82; RRID: AB_11151330
BV786 mouse anti-human CD69	BD Biosciences	Cat # 563834; RRID: AB_2738441
PE-Cy7 mouse anti-human IFN-γ	BD Biosciences	Cat # 557643; RRID: AB_396760
Annexin V- PE	BD Biosciences	Cat # 556421; RRID: AB_2869071
7-AAD	BD Biosciences	Cat # 559925; RRID: AB_2869266
p-ATM S1981	Cell Signaling Technology	Cat #5883
p-ATR T1989	Cell Signaling Technology	Cat #30632; RRID: AB_2798992
p-Chk1 S317	Cell Signaling Technology	Cat #12302; RRID: AB_2893473
p-Chk2 T68	Cell Signaling Technology	Cat #2197; RRID: AB_331479
GAPDH	Cell Signaling Technology	Cat #2118; RRID: AB_561053
Peroxidase AffiniPure goat anti-rabbit IgG (H + L)	Jackson Immunoresearch	Cat #111-035-144; RRID: AB_2307391
Biological samples
IMvigor130 mUC samples	Genentech	N/A
IMvigor130 PBMC collection for CITE-seq (n = 113)	This study	N/A
Chemicals, peptides, and recombinant proteins
Cisplatin	Sigma Aldrich	CAS#: 15663-27-1
Carboplatin	Sigma Aldrich	CAS#: 41575-94-4
KU-55933 (ATM Kinase Inhibitor)	Selleckchem	Cat#: S1092
VE-821(ATR inhibitor IV)	Selleckchem	Cat#: S8007
Halt protease and phosphatase inhibitor cocktail, EDTA-free	Thermo Fisher	Cat#: 78441
Critical commercial assays
10x Chromium Next GEM Chip G Kit	10X Genomics
Pan T cell Isolation Kit, human	Miltenyi Biotec	Cat# 130-096-535
Dynabeads Human T-Activator CD3/CD28 for T cell expansion and activation	Thermo Fisher	Cat# 11161D
CD14 MicroBeads, human	Miltenyi Biotec	Cat# 130-050-201
CellXVivo human monocyte-derived DC differentiation kit	R&D	Cat# CDK004
Cytiva Ficoll-Paque PLUS Media	Fisher Scientific	Cat# 45-001-749
Human IFNg ELISA kit	BD Biosciences	Cat.# 555142
Mouse CXCL10 ELISA kit	Thermo Fisher Scientific	Cat# BMS6018
Mouse HMGB1 ELISA kit	Tecan	Cat# 30164033
CellTrace Blue Cell Proliferation kit	Thermo Fisher Scientific	Cat# C34568
RNeasy kits	Qiagen
FITC Annexin V Apoptosis Detection Kit with 7-AAD	BD Biosciences	Cat#: 556547
RIPA buffer	Sigma
LIVE/DEAD Fixable Aqua Dead Cell Stain Kit	Invitrogen	L34957
Deposited data
Raw and analyzed bulk RNAseq (cell lines)	This paper	GEO: GSE235066
PBMC scRNA-seq raw counts and metadata	This paper	EGAS50000000104
IMvigor130 bulk RNAseq processed data (patient tumors)	This paper	EGAS50000000104
Experimental models: Cell lines
Human cell line: 5637	Genentech	N/A
Human cell line: RT112	Genentech	N/A
Mouse cell line: MC38	Genentech	N/A
Mouse cell line: MC38-OVA	Genentech	N/A
Experimental models: Organisms/strains
C57BL/6
C57BL/6J.OT-I.Thy1.1
Software and algorithms
R statistical software, version 4.0.2	The R Foundation	https://www.r-project.org/
GSNAP	Wu et al., 201649; Wu et al., 201050
survminer package, version 0.4.8	CRAN Repository	https://cran.r-project.org/web/packages/survminer/index.html
survival package, version 3.2–7	CRAN Repository	https://cran.r-project.org/web/packages/survival/index.html
limma package, 3.44.3	Bioconductor	https://bioconductor.org/packages/release/bioc/html/limma.html
fgsea package, 1.14.0	Bioconductor	http://bioconductor.org/packages/fgsea
CellRanger, 3.1.0	10X Genomics	http://10xgenomics.com/
Seurat, v3.2.2	Stuart et al., 201951	https://satijalab.org/seurat
Harmony, v1.0	Korsunsky et al., 201952	https://github.com/immunogenomics/harmony
FlowJo	Becton Dickinson	https://flowjo.com/
Graphpad Prism 7.0 software	GraphPad Software, Inc.	http://www.graphpad.com/scientificsoftware/prism/
GSVA	Bioconductor	https://bioconductor.org/packages/release/bioc/html/GSVA.html