REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
anti-human CD3-PE-Cy7	BioLegend	Cat#300420, RRID: AB_439781
APC/Cyanine7 Mouse IgG1, κ isotype Ctrl	Biolegend	Cat#400128; RRID: AB_2892538; RRID
APC Mouse IgG1, κ isotype Ctrl	Biolegend	Cat#400120; RRID: AB_2888687
PE/Cyanine7 Mouse IgG1, κ isotype Ctrl	Biolegend	Cat#400126; RRID: AB_2861433
Brilliant Violet 605™ Mouse IgG1, κ Isotype Ctrl	Biolegend	Cat#400162; RRID: AB_11125373
FITC Mouse IgG1, κ Isotype Ctrl	Biolegend	Cat#400108; RRID: AB_326429
Brilliant Violet 421™ Mouse IgG1, κ Isotype Ctrl	Biolegend	Cat#400158; RRID: AB_11150232
PE Mouse IgG1, κ isotype Ctrl	Biolegend	Cat#400112; RRID: AB_3076354
anti-human CD69-FITC	BioLegend	Cat#310904; RRID: AB_314839
anti-human PD1-APC	BioLegend	Cat#329908; RRID: AB_940475
anti-human TIM-3-PE	BioLegend	Cat#345006; RRID:AB_2116576
anti-human LAG-3-PE-Cy7	BioLegend	Cat#369208; RRID: AB_2629834
anti-human CD62L-PE	BioLegend	Cat#304806; RRID: AB_314465
anti-human CD45RO-APC	BioLegend	Cat#304210; RRID: AB_314426
anti-human IL-2-APC	BioLegend	Cat#500310; RRID: AB_315096
anti-human TNFα-PE	BioLegend	Cat#502909; RRID: AB_315261
anti-human Granzyme B-PE	BioLegend	Cat#372208; RRID: AB_2687031
anti-human IFNγ-APC	BioLegend	Cat#502512; RRID: AB_315237
anti-human Annexin-V-APC	BioLegend	Cat#640920; RRID: AB_2941659
anti-human PD1-BV421	BD Biosciences	Cat#564323; RRID: AB_10900818
anti-human GLUT1 mAb	Abcam	Cat#ab115730; RRID: AB_10903230
anti-human GLUT3 mAb	Abcam	Cat#ab191071; RRID: AB_2736916
anti-human CD38 mAb	Abcam	Cat#ab108403; RRID: 10890803
Chemicals, peptides, and recombinant proteins
78C	Selleck	Cat#S8960
RBN013209	MCE	Cat#HY-144987
Luteolinidin Chloride	Targetmol	Cat#TN1895
8-Bromo-cADP-Ribose (8-Br-cADPR)	SANTA	Cat#sc-201514A
cADP-Ribose (cADPR)	Sigma	Cat#C7344
IOX2	Selleck	Cat#S2919
Adenosine 5′-diphosphoribose sodium (ADPR sodium)	MCE	Cat#HY-100973A
Nicotinic acid adenine dinucleotide phosphate sodium salt (NAADP sodium)	sigma	Cat#N5655
Dehydronitrosonisoldipine	MCE	Cat#HY-Z0816
DSRM-3716	MCE	Cat#HY-W021879
Ned19	Targetmol	Cat#T12205
Critical commercial assays
Cell Counting Kit-8 (CCK-8)	DOJINDO	Cat#CK04
MitoProbe™ DiIC1(5) Assay Kit	Invitrogen	Cat#M34151
Fluo-4 a.m. ester	Thermo Fisher	Cat#F14201
RNA Isolation Kit	Qiagen	Cat#931636
Pierce BCA Protein Assay Kit	Thermo Scientific	Cat#23225
Cytoplasmic Extraction Reagents Kit	Thermo Scientific	Cat#78833
ELISA kit	MLUTI SCIENCES	Cat#E2620
Seahorse XF Glycolytic Stress Assay Kit	Agilent	Cat#103020-100
Seahorse XF Mito Stress Assay Kit	Agilent	Cat#103015-100
NAD+/NADH ratio Assay Kit	Beyotime	Cat#S0175
Universal Sirt1 Activity Assay Kit	Abcam	Cat#ab156065
by Nicotinic Acid Adenine Dinucleotide Phosphate (NAADP) ELISA Kit	MyBioSource	Cat#MBS2000162
Deposited data
Bulk RNA-seq data in this paper	Genome Sequence Archive in China National Genomics Data Center	GSA:HRA004078
scRNA-seq data	Gene Expression Omnibus	GEO: GSE197268
scATAC-seq data	China National GeneBank DataBase (CNGBdb)	CNP0002442
Experimental models: Cell lines
293T cell line	ATCC	Cat#CRL-1573
Nalm6 cell line	ATCC	Cat#CRL-3273
143B cell line	ATCC	Cat#CRL-8303
Experimental models: Organisms/strains
Mouse: NOD-SCID-Il2rg−/− (NSG)	Shanghai Model Biological Center	N/A
Oligonucleotides
Primers	See Table S1 for primers and RNA sequences.	N/A
Recombinant DNA
Anti-CD19 scFv (FMC63)	Auther W. Hohman lab	N/A
Anti-GD2 scFv (14g2a-E101K)	Carl June lab	N/A
pLV-shRNA-CAR	this manuscript	N/A
Software and algorithms
Signac 1.10.0	https://doi.org/10.1038/s41592-021-01282-5	https://stuartlab.org/signac/
FlowJo 10.0	FlowJo, LLC	https://www.flowjo.com/
Graphpad Prism version 10.0	GraphPad software	https://www.graphpad.com
ChIPseeker R package	https://doi.org/10.1002/cpz1.585	https://bioconductor.org/packages/ChIPseeker/
R version 4.2.0	R Core Team 2017	https://www.r-project.org
Seraut	Rahul Satija Lab	https://github.com/satijalab/seurat
GO	GO Consortium	http://geneontology.org/
GSEA	Broad Institute	https://www.gsea-msigdb.org/gsea/index.jsp
Clusterprofiler	Guangchuang Yu lab	http://bioconductor.org/packages/release/bioc/html/clusterProfiler.html
HISAT2 version 2.0.4	https://doi.org/10.1038/nmeth.3317	http://www.ccb.jhu.edu/software/hisat/
StringTie version 1.3.4days	https://doi.org/10.1038/nbt.3122	https://github.com/gpertea/stringtie
edgeR	https://doi.org/10.1093/bioinformatics/btp616	https://bioconductor.org/packages/release/bioc/html/edgeR.html