Figure 7.

FOLR2⁺ macrophage depletion disrupts the immunosuppressive tumor microenvironment (TME)

(A) The workflow of the in vivo experiment.

(B) The tumor burden from mice of four groups was assessed (n = 5).

(C) The statistics for tumor burden in each group. Significance was evaluated by a Kruskal-Wallis test (n = 5).

(D) H&E and Ki-67 IHC staining for the tumor tissues from four groups of mice (biological replicates, n = 5). Scale bars, 100 μm (H&E) and 40 μm (IHC)

(E) The statistics for Ki-67-positive cells in each group. Significance was evaluated by a Kruskal-Wallis test (n = 5).

(F) Multiplex immunohistochemistry staining of F4/80 (green) and FOLR2 (red) on tumor tissues in the in vivo model.