Figure 6.
Immunofluorescence detection of Mas-related G protein-coupled receptor member D (MrgD) in the lungs of rats
Triple immunostaining was performed to visualize the MrgD receptor (green) and nucleus with DAPI (blue). Representative images showed the expression of MrgD in the lung membrane. Quantitative analysis revealed a higher expression of MrgD in the monocrotaline (MCT)-induced pulmonary hypertension group compared to the control group. However, a decreased expression of MrgD was observed in the MCT + alamandine group compared to the MCT group. The significance levels were denoted as **P<0.01, ***P<0.001, and P<0.05.