REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-human CD3 (clone UCHT1)	BD Biosciences	Cat# 563546; RRID:AB_2744387
Anti-human CD8 (clone SK1)	BD Biosciences	Cat# 561617, RRID:AB_10896281
Anti-human CD16 (clone 3G8)	BD Biosciences	Cat# 563689; RRID:AB_2744299
Anti-human CD33 (clone P67.6)	BD Biosciences	Cat# 333946; RRID:AB_399961
Anti-human CD40 (clone 5C3)	BD Biosciences	Cat# 561211; RRID:AB_10584325
Anti-human CD80 (clone L307.4)	BD Biosciences	Cat# 560926; RRID:AB_10565975
Anti-human CD83 (clone HB15e)	BD Biosciences	Cat# 565336; RRID:AB_2739191
Anti-human CD86 (clone 2331)	BD Biosciences	Cat# 563460; RRID:AB_2744455
Anti-human CSF1R (clone 9-4D2-1E4)	BD Biosciences	Cat# 564945; RRID:AB_2739022
Anti-human CXCR4 (clone 12G5)	BD Biosciences	Cat# 555976; RRID:AB_398616
Anti-human B7-H4 (clone MIH43)	BD Biosciences	Cat# 562787; RRID:AB_2737794
Anti-human VISTA (clone MIH65.rMab)	BD Biosciences	Cat# 566669; RRID:AB_2739762
Anti-human IFNγR1(clone GIR208)	BD Biosciences	Cat# 558934; RRID:AB_397163
Anti-human pSTAT1 (clone pY701; 4a)	BD Biosciences	Cat# 612596; RRID:AB_399879
Anti-human pSTAT3 (clone pS727; 49/p-Stat3)	BD Biosciences	Cat# 558099; RRID:AB_397024
Anti-human pSTAT5 (clone pY694; 47/Stat5)	BD Biosciences	Cat# 560117; RRID:AB_1645546
Anti-human pmTOR (clone pS2448; O21-404)	BD Biosciences	Cat#563489; RRID:AB_2736872
Anti-human pAkt (clone pS473; M89-61)	BD Biosciences	Cat# 560378; RRID:AB_1645328
Anti-human PD-L1 (clone 29E.2A3)	BioLegend	Cat# 329714; RRID:AB_2563852
Anti-human CD14 (clone HCD14)	BioLegend	Cat# 325617; RRID:AB_830690
Anti-human PD-L2 (clone MIH18)	BioLegend	Cat# 345515; RRID:AB_2783233
Anti-human B7-H3 (clone MIH42)	BioLegend	Cat# 351010; RRID:AB_2728323
Anti-human CD45 (clone HI30)	BioLegend	Cat# 304043; RRID:AB_2562498
Anti-human HLA-DR (clone LN3)	BioLegend	Cat# 327007; RRID:AB_893579
Anti-human CD54 (clone HA58)	BioLegend	Cat# 353108; RRID:AB_10900254
Anti-human CD69 (clone FN50)	BioLegend	Cat# 310932; RRID:AB_2563696
Anti-human MRC1 (clone 15-2)	BioLegend	Cat# 321122; RRID:AB_10899411
Anti-human CD163 (clone GHI/61)	BioLegend	Cat# 321122; RRID:AB_10899411
Anti-human CD32 (clone FUN-2)	BioLegend	Cat# 303229; RRID:AB_2894576
Anti-human CD64 (clone 10.1)	BioLegend	Cat# 305028; RRID:AB_2563822
Anti-human CCR2 (clone K036C2)	BioLegend	Cat# 305028; RRID:AB_2563822
Anti-human CCR5 (clone J418F1)	BioLegend	Cat# 359145; RRID:AB_3068173
Anti-human PD-L1 (SP142)	Abcam	Cat# ab228462; RRID:AB_2827816
Anti-human CD3 (clone LN10)	Biocare Medical	Cat#3152
Anti-human CD8 (clone SP16)	Biocare Medical	Cat#CRM 311; RRID:AB_2750579
Anti-human CD68 (clone KP1)	Biocare Medical	Cat#033; RRID:AB_2885063
Anti-human Cytokeratin (clone AE1/AE3)	Biocare Medical	Cat#CM 011; RRID:AB_2811020
LIVE/DEAD Fixable Blue	ThermoFisher	Cat#L23105
Chemicals, peptides, and recombinant proteins
ERK1/2 inhibitor SCH772984	Selleck	Cat# S7101
STAT1 inhibitor Fludarabine	Selleck	Cat# S1491
Akt1/2/3 inhibitor MK-2206 2HCl	Selleck	Cat# S1078
PI3Kα/δ/β inhibitor LY294002	Selleck	Cat# S1105
NF-κB inhibitor QNZ	Selleck	Cat# S4902
mTOR inhibitor Rapamycin	Selleck	Cat# S1039
Critical commercial assays
pHrodo phagocytosis assay	ThermoFisher	Cat# P35366
Deposited data
Single-Cell RNA-sequencing data – In-house dataset	This paper	GSE248288
Software and algorithms
Flowjo v10	Flowjo software	https://www.flowjo.com/
Graphpad Prism v9	Graphpad software	https://www.graphpad.com/
EndNote X8	Endnote software	https://endnote.com/
BioRender	BioRender website	https://www.biorender.com/
QuPath	QuPath software	https://doi.org/10.1038/s41598-017-17204-5
R	R Core Team	https://www.R-project.org/
Python	Python Software Foundation	http://www.python.org
Seurat	Stuart et al.54	https://doi.org/10.1016/j.cell.2019.05.031
icellnet	Noël et al.55	https://doi.org/10.1038/s41467-021-21244-x
CellPhoneDB V2.0	Efremova et al.56	https://github.com/ventolab/CellphoneDB
Customized scRNA-seq analysis pipeline	This paper	https://doi.org/10.5281/zenodo.10472431