REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
CD19 CAR FMC63 Idiotype Antibody, REAfinity™, Biotin	Miltenyi Biotec	Cat#130-127-349; RRID:AB_2923109
Biotin Antibody, PE, REAfinity™	Miltenyi Biotec	Cat#130-110-951; RRID:AB_2661378
Brilliant Violet 421(TM) anti-human CD152 (CTLA-4)	BioLegend	Cat#369605; RRID:AB_2616790
CD3 Antibody, anti-human, APC	Miltenyi Biotec	Cat#130-113-125; RRID:AB_2725953
Anti-CD19 APC (LT19)	ImmunoTools	Cat#21270196; RRID:AB_2923108
Rabbit Anti-TEM1/CD248 Polyclonal Antibody, FITC Conjugated	Bioss	Cat#bs-2101R-FITC; RRID:AB_11083094
Anti-human CD152 (CTLA-4) PE/Cyanine7	BioLegend	Cat#369614; RRID:AB_2632876
Anti-human CD3 BV421	BioLegend	Cat#317344; RRID:AB_2565849
Goat Anti-mouse IgG Fab-Biotin	SouthernBiotech	Cat#1015-08; RRID:AB_2794195
Horse Anti-mouse HRP	Cell Signaling Technologies	Cat#7076S; RRID:AB_330924
Anti-CD20 APC (LT20)	ImmunoTools	Cat#21279206; RRID:AB_2923110
CD80 (B7-1) Monoclonal Antibody (2D10.4), Biotin, eBioscience	Thermo Fisher Scientific	Cat#13-0809-82; RRID:AB_466513
B7-2/CD86 Antibody (SPM600)	Novus Biologicals	Cat#NBP2-44515; RRID:AB_2923113
CD3 Monoclonal Antibody (UCHT1), Alexa Fluor™ 532, eBioscience	Thermo Fisher Scientific	Cat#58-0038-42; RRID:AB_11218675
Alexa Fluor® 488 Streptavidin	BioLegend	Cat#405235
Alexa Fluor® 647 anti-human CD19	BioLegend	Cat#302220; RRID:AB_389335
B7-2/CD86 Antibody (BU63)	Novus Biologicals	Cat#NBP2-25208; RRID:AB_2923115
Mouse Anti-IFN-gamma Monoclonal Antibody, Unconjugated, Clone NIB42	BD Biosciences	Cat#551221; RRID:AB_394099
Mouse Anti-IL-2 Monoclonal Antibody, Unconjugated, Clone 5344.111	BD Biosciences	Cat#555051; RRID:AB_395672
Mouse Anti-IFN-gamma Monoclonal Antibody, Biotin Conjugated, Clone 4S.B3	BD Biosciences	Cat#554550; RRID:AB_395472
Mouse Anti-IL-2 Monoclonal Antibody, Biotin Conjugated, Clone B33-2	BD Biosciences	Cat#555040; RRID:AB_395666
Anti-APC MicroBeads	Miltenyi Biotec	Cat#130-090-855; RRID:AB_244367
CD3 Antibody, anti-human, PE, REAfinity™	Miltenyi Biotec	Cat#130-113-139; RRID:AB_2725967
Brilliant Violet 510™ anti-human CD5 Antibody	BioLegend	Cat#364018; RRID:AB_2565728
CD19 Antibody, anti-human, FITC, REAfinity™	Miltenyi Biotec	Cat#130-113-645; RRID:AB_2726198
APC/Fire™ 750 anti-human CD20 Antibody	BioLegend	Cat#302357; RRID:AB_2572125
Brilliant Violet 421™ anti-human CD80 Antibody	BioLegend	Cat#305222; RRID:AB_2564407
anti-human CD86 APC-conjugated	ImmunoTools	Cat#21480866; RRID:AB_2923116
anti-human CD80 PE-conjugated	ImmunoTools	Cat#21270804; RRID:AB_2923118
B7-2/CD86 Antibody (BU63) [Alexa Fluor® 532]	Novus Biologicals	Cat# NBP2-34569AF532; RRID:AB_2923133
PE anti-mouse CD80 Antibody, Clone16-10A1	BioLegend	Cat#104707; RRID:AB_313128
PE/Cyanine7 anti-mouse CD86, Clone GL-1	BioLegend	Cat#105014; RRID:AB_439783
APC anti-mouse CD152 Antibody, Clone UC10-4B9	BioLegend	Cat#106309; RRID:AB_2230158
Ultra-LEAF Purified anti-mouse CD28 Antibody, Clone 37.51	BioLegend	Cat#102116; RRID:AB_11147170
Ultra-LEAF(TM) Purified anti-mouse CD3epsilon antibody, Clone145-2C11	BioLegend	Cat#100340; RRID:AB_11149115
Brilliant Violet 421(TM) anti-mouse CD3epsilon antibody, Clone1452C11	BioLegend	Cat#100341; RRID:AB_2562556
Anti-mouse CD19 APC, Clone 1D3	Immunotools	Cat#22270196; RRID:AB_2938950
Biotin-SP-AffiniPure F(ab')2 Fragment Goat Anti-Rat IgG, F(ab')2 Fragment Specific (min X Hu,Bov,Hrs Sr Prot) antibody	Jackson ImmunoResearch Labs	Cat#112-066-072; RRID:AB_2338185
Brilliant Violet 510(TM) anti-mouse CD19 antibody, Clone 6D5	BioLegend	Cat#115545; RRID:AB_2562136
IFN gamma Monoclonal Antibody (AN-18), eBioscience, Clone AN-18	Thermo Fisher Scientific	Cat#14-7313-81; RRID:AB_468471
Biotin anti-Mouse IFN gamma	BD Pharmingen	Cat#551506; RRID:AB_394224
anti-p-IKK alpha/beta (S176 + S180)	Bioss	Cat#bs-3237R; RRID:AB_10883648
anti-Rabbit IgG HRP	Santa Cruz Biotechnology	Cat#sc-2030; RRID:AB_631747
Goat F(ab')2 Anti-Human IgG, Mouse ads-PE	Southern Biotech	Cat#2043-09; RRID:AB_2795669
Purified anti-human CD19 Antibody	BioLegend	Cat#302202; RRID:AB_314232
Biotin anti-mouse IgG1 Antibody	BioLegend	Cat#406603; RRID:AB_315062
G4S Linker (E7O2V) Rabbit mAb (Alexa Fluor ® 488 Conjugate)	Cell Signaling Technologies	Cat#50515S; RRID:AB_2941670
PE anti-human CD25	BioLegend	Cat#302606; RRID:AB_314276
CD3 Antibody, anti-human, FITC, REAfinity™	Miltenyi Biotec	Cat#130-113-138; RRID:AB_2725966
Brilliant Violet 510™ anti-human CD4	BioLegend	Cat#317444; RRID:AB_2561866
CD8 Antibody, anti-human, APC-Vio® 770, REAfinity™	Miltenyi Biotec	Cat#130-110-819; RRID:AB_2659246
Brilliant Violet 421™ anti-human CD3	BioLegend	Cat#317344; RRID:AB_2565849
APC anti-human CD27	BioLegend	Cat#356410; RRID:AB_2561957
PE/Fire™ 700 anti-human CD45RA	BioLegend	Cat#304171; RRID:AB_2888784)
FITC anti-human CD45RO	BioLegend	Cat#304204; RRID:AB_314420
APC/Fire™ 750 anti-human CD62L	BioLegend	Cat#304845; RRID:AB_2629675
PE anti-human CD4	BioLegend	Cat#317410; RRID:AB_571955
FITC anti-human CD8a	BioLegend	Cat#300906; RRID:AB_314110
PE/Cyanine7 anti-human TIGIT (VSTM3)	BioLegend	Cat#372713; RRID:AB_2632928
APC anti-human CD279 (PD-1)	BioLegend	Cat#329908; RRID:AB_940475
APC/Fire(TM) 750 anti-human CD366 (Tim-3)	BioLegend	Cat#345043; RRID:AB_2632855
Goat F(ab')2 Anti-Human IgG, Mouse ads-FITC	SouthernBiotech	Cat#2043-02; RRID:AB_2795666
Chemicals, peptides, and recombinant proteins
Recombinant Human Transforming Growth Factor β 3	ImmunoTools	Cat#11344485
PEIpro®	Polyplus	Cat#101000026
Recombinant Human B7-2 Fc	Peprotech	Cat#310-33
Propidium Iodide Solution	Miltenyi Biotec	Cat#130-093-233
CellTrace Violet Cell Proliferation Kit	Invitrogen	Cat#C34557
Streptavidin-PE, SAv-PE	BioLegend	Cat#405203
7-AAD Viability Staining Solution	BioLegend	Cat#420404
Streptavidin-POD Conjugate	Roche Diagnostics	Cat#11089153001
Streptavidin FITC-conjugated	ImmunoTools	Cat#31274243
Recombinant Human CD19-Fc Chimera	BioLegend	Cat#789006
Human B7-1/CD80 Protein, Fc Tag (MALS verified)	Acro Biosystems	Cat#B71-H5259
Human B7-2/CD86 Protein, Fc Tag, premium grade	Acro Biosystems	Cat#CD6-H5257
Critical commercial assays
ELISA MAX™ Deluxe Set Human IL-6	BioLegend	Cat#430504
MojoSort Mouse CD3 T cell Isolation Kit	BioLegend	Cat#480031
MojoSort Mouse Pan B Cell Isolation Kit II	BioLegend	Cat#480087
CellTrace™ Cell Violet Proliferation Kit	Invitrogen	Cat#C34557
Experimental models: Cell lines
Human: Raji	DSMZ	Cat#ACC 319; RRID:CVCL_0511
Human: SU-DHL-10	DSMZ	Cat#ACC 576; RRID:CVCL_1889
Human: DOHH-2	DSMZ	Cat#ACC 47; RRID:CVCL_1179
Human: Oci-Ly1	DSMZ	Cat#ACC 722; RRID:CVCL_1879
Human: Oci-Ly19	DSMZ	Cat#ACC 528; RRID:CVCL_1878
Human: HEK-293	DSMZ	Cat#ACC 305; RRID:CVCL_0045
Human: Vascular Wall-Typical Mesenchymal Stem Cells (Hita08/20P5)	Diana Klein39	N/A
Murine: Prdm1.fl/Myd88/Bcl2-derived lymphoma cell line	Ruth Flümann40	N/A
Experimental models: Organisms/strains
Mouse: Rag2tm1.1Flv Il2rgtm1.1Flv (Rag2- γc-)	The Jackson Laboratory	Cat#014593; RRID:IMSR_JAX:014593
Mouse: Prdm1fl/fl; Myd88cond.p.L252P/wt;Rosa26LSL.BCL2.IRES.GFP/wt;Cd19Cre/wt (Prdm1.fl/Myd88/Bcl2)	Ruth Flümann40	N/A
Mouse: C57BL/6J (Black 6)	The Jackson Laboratory	RRID:IMSR_JAX:000664
Recombinant DNA
Plasmid: pBullet_aCD19_CD3zeta_P2A_CTLA4_4-1BB	This paper	N/A
Plasmid: pBullet_aCD19_CD3zeta	This paper	N/A
Plasmid: pBullet_aCD19_CD3zeta_4-1BB	This paper	N/A
Plasmid: pBullet_aCD20_CD3zeta_4-1BB	This paper	N/A
Plasmid: pBullet_aCD20_CD3zeta_P2A_CTLA-4_4-1BB	This paper	N/A
Plasmid: pBullet_aCD19_CD3zeta_P2A_aCD86_4-1BB	This paper	N/A
Plasmid: pQCXIP_eGFP	This paper	N/A
Plasmid: pQCXIP_fLuc_tdTomato	This paper	N/A
Software and algorithms
R code	This paper	https://doi.org/10.5281/zenodo.10342282
RStudio	RStudio Team	https://www.rstudio.com/
R Core	R Core Team	https://www.r-project.org/
ggpubr		https://cran.r-project.org/web/packages/ggpubr/index.html
Survival		https://cran.r-project.org/web/packages/survival/index.html
Survminer		https://cran.r-project.org/web/packages/survminer/index.html
rstatix		https://cran.r-project.org/web/packages/rstatix/index.html
Other
Mesenchymal Stem Cell Growth Medium 2	PromoCell	Cat#C-28009
Canto II Flow Cytometer	Becton Dickinson	https://www.bdbiosciences.com/en-eu/products/instruments/flow-cytometers/clinical-cell-analyzers/facscanto
Multiskan GO Microplate Spectrophotometer	Thermo Scientific	https://www.fishersci.de/shop/products/multiskan-go-microplate-spectrophotometer/p-4530546
Hidex Sense Microplate Reader	Hidex	https://www.hidex.de/hidex-sense/
IVIS 200 Spectrum In Vivo Imaging System	PerkinElmer	https://www.perkinelmer.com/de/product/ivis-instrument-spectrum-120v-andor-c-124262
FV 1000 confocal laser scanning microscope	Olympus	https://www.olympus-lifescience.com/de/technology/museum/micro/2004/
UC90 4K microscope	Olympus	https://www.olympus-lifescience.com/de/camera/color/uc90/
BX53 microscope	Olympus	https://www.olympus-lifescience.com/en/microscopes/upright/bx53f2/
Fusion Solo S	Vilber	https://www.vilber.com/fusion-solo-s/
MACSQuant X	Miltenyi Biotec	https://www.miltenyibiotec.com/US-en/products/macsquant-x.html