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T antigen stimulates the formation of the TA complex on the Sp1-hsp70 TATA promoter. Various combinations of purified TBP, TFIIA, TFIIB, and T antigen (Tag) (as indicated; also described in Materials and Methods) were incubated with a 32P-labeled Sp1-hsp70 TATA promoter fragment. The complexes formed were analyzed by EMSA.
