Fig. 2: Effects of antidepressants and diethyldithiocarbamic acid (DETC) on hydrogen peroxide-induced neurotoxicity in PC12 cells. Before being exposed to 200 μmol/L H₂O₂ for 4 hours, cells were pretreated with (A) 100 μmol/L amitriptyline (AMI) or 100 μmol/L fluoxetine (FLU) for 24 hours; (B) 50 μmol/L amitriptyline or 50 μmol/L fluoxetine for 48 hours; or (C) 50 μmol/L fluoxetine or 50 μmol/L fluoxetine plus 1.0 mmol/L DETC (D) for 48 hours. Data are presented as mean (and standard error of the mean) from 4 independent experiments. *p < 0.001 v. vehicle control (VEH); †p < 0.01 v. 200 μmol/L H₂O₂ treatment alone; ‡p < 0.001 v. 200 μmol/L H₂O₂ treatment alone.