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Binding for all CSS.3 aptamer truncations was measured by using BLI. (a) Calculated binding affinities from steady state equilibrium to cortisol in sISF. (b) Replicate traces showing real-time baseline, association and dissociation and (c) averaged steady state binding traces for stem-optimized CSS.3_cut3 aptamer are featured. SD = standard deviation. Experiments were conducted at 30 °C in sISF.
