Figure 1. Generation and characterization of Ubap2 knock-out mice.

(A) Schematic representation of the targeting strategy. Mouse line that carries a floxed Ubap2 (exon 5) coding region flanked by two loxP-recognition sites for Cre recombinase. Flp recombinase-activity led to deletion of the selection cassette resulting in a Ubap2 floxed allele (Ubap2^flox). (B) PCR analysis of the transgenic mice from the DNA isolated from the tail snip. PCR of exon five region showed a 250bp fragment for the wild-type allele while the Ubap2^flox allele was 415bp in size. For Pdx-1-Cre, PCR amplification resulted in 650 bp, while the internal positive control amplified the wild-type Pdx gene at 415 bp. (C) H&E Staining of pancreatic tissue sections in Ubap2^flox/flox and U2KO animals. (D) DNA analysis of Ubap2 from whole-cell lysates of the pancreas (N=10).