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. Author manuscript; available in PMC: 2024 Dec 1.
Published in final edited form as: Cancer Lett. 2023 Oct 19;578:216455. doi: 10.1016/j.canlet.2023.216455

Figure 7. NF-κB signaling is reduced in U2KO mice when challenged with cerulein.

Figure 7.

(A) NF-κB immunofluorescence staining in U2KO mice and control animals. Pancreatic tissue was taken from animals after Cerulein (50ug/kg) or an equivalent volume of PBS as a control (Figure S2B). Nuclei are stained with DAPI (blue). (B) Quantification of 40X magnification images from U2KO animals or control animals. Graph was generated from the number of positive cells within a single image field of a slide. 8 images are taken per animal and 4 animals were used per group analyzed. (C) Chromatin immunoprecipitation assays in AR42J cells were after 150nM cerulein for 24 hours or an equivalent volume of PBS. Cells were transiently transfected with either a nontargeting scramble siRNA or an siRNA targeting Ubap2. DNA gels were run from PCR-amplified products targeting the IL-1β promoter or the IκBα promoter region. Actin is used as a control for DNA loading. Comparisons were made with the Student’s T-test. Data are expressed as mean ± standard error of the mean (SEM), n=5–7. All analyses were performed through GraphPad-PRISM (7.04) (N.S. = Not significant).