Fig. 1.

Comprehensive proteotranscriptomic profiling of the hypoxic surfaceome and endocytome in GBM. A Three primary GBM cell cultures representative of different molecular subtypes (MS mesenchymal, CL classical, and PN proneural) as well as the glioma cell line, U87MG, were cultured in normoxic or hypoxic conditions. B TS-MAP workflow for enrichment of the surfaceome and endocytome. C Quantitative LC–MS/MS of TS-MAP-enriched proteins and RNAseq from normoxic and hypoxic cultures. Data was filtered for bona fide plasma membrane proteins by the SURFME classifier. D Validation of target candidates by IF analyses of normoxic and hypoxic 2D cultures, spheroid (3D) hypoxic core, and comparative stainings of GBM and low-grade glioma (LGG; diffuse astrocytoma, WHO grade II) tumors, followed by selection for in vitro ADC treatment studies